The Viral Vector Core facility is located in the Princeton Neuroscience Institute. Our main objective is to design and provide investigators with high quality viral and non-viral vector tools that can be used in vitro and in vivo. Our services include:
- Production of highly concentrated recombinant adeno-associated virus (rAAV).
- Production of replication competent pseudorabies virus (PRV) and herpes simplex virus (HSV-1).
- Production of replication incompetent gammaretrovirus (RV).
- Consultation in the design of custom viruses and preparation of plasmids required for virus production.
- Custom cloning.
Esteban Engel, PhD Director of the Viral Neuroengineering Laboratory eengel [at] princeton.edu Office A50 Angela Chan, BSc Research Specialist II achan [at] princeton.edu Office A76C
Angela received her BA in Biology at Hood College, Maryland. After graduation, she worked in the Monoclonal Antibody Facility at Princeton University for 10 years. Later, she worked in the lab of Dr. M. Gerard Waters in the Department of Molecular Biology, where she purified and made polyclonal antibodies to Dsl3p. For the past 17 years, she has been the lab manager and a researcher in the lab of Dr. Virginia Zakian. There she used ChIP and genetic approaches to determine the mechanism of telomerase recruitment and regulation and investigated the roles of the Pif1 DNA helicase at telomeres. Her work has been published in several journals. She joined the Viral Core Facility at PNI in 2019.
Oliver Huang, PhD Research Specialist II haoh [at] princeton.edu Office A76D
Oliver received his B.A. in Molecular and Cell Biology at University of California, Berkeley, with the emphasis on immunology. For his thesis work, he demonstrated the activation of innate immune pathway P38 MAPK by gut microbiota in C. elegans. He further received his PhD in Virology under the mentorship of Prof Lynn Enquist, studying virus-induced degradation of kinesin-3 motor KIF1A in neuronal infection of an alphaherpesvirus, pseudorabies virus. Oliver joined the Viral Core Facility in August, 2020