Recombinant adeno-associated virus safety and handling

  • Recombinant AAV stocks are provided in a phosphate buffered saline (PBS) buffer with 35mM sodium chloride, 0.002% pluronic F-68 and 5% glycerol. Do not dilute the sample into low salt buffer or water.
  • Store at -800C with minimal freeze-thaw cycles.
  • Avoid vortex or excessive pipetting of the sample.
  • Do not dry the sample.
  • Do not expose the sample to extreme conditions such as very low or high pH, high temperature, denaturant agents, detergents, organic compounds, etc.
  • Do not transfer between tubes more than necessary, especially when using regular hydrophobic plastics. If possible use siliconized or similar low protein binding tubes.
  • Recombinant AAV vectors are replication incompetent, the transgene rarely integrates into the host genome.
  • NIH and OSHA guidelines state that replication-defective rAAVs that do not contain a tumorigenic gene product or a toxin can be handled at Biosafety level 1 (BSL-1). To disinfect material exposed to AAV you can use 0.5% sodium hypochlorite, 2% gutaraldehyde, or autoclave for 30 minutes. Ethanol is not an effective disinfection agent.
  • The Principal Investigator of the recipient laboratory assumes safety and handling responsibility once the sample leaves our premises.